Hi everybody,
for some mysterious reason, I'm able to post here but can't read in
bionet.molbio.proteins.fluorescent. I'd be grateful if you could reply
by mail. Maybe someone can tell me where I can find the FAQ?
anyway, my question is about the pGFP-N3 N-terminal protein fusion
vector (Clontech). To put the problem simple: IT DOESN'T WORK. No
matter what, lower temperatures, different celltypes....even the
control doesn't work.
Who has the same (or even better: the opposite) results with the same
vector and knows what the problem is (or even better: was).
any aditional information on GFP fusion protein systems are very
welcome!
please use my mail address: petra at emsaserv.biol.ruu.nl , since I'm
using somebody else's box right now (it's about time our network is
fixed!)
Thanx a zillion in advance,
Petra de Graaf
