gfp in plants

Robert Lamarche robert at bio1.lan.mcgill.ca
Tue Mar 12 03:28:12 EST 1996

We have introduced Jim Haseloff's 35S-GFP construct into plants.  Several
independent transformants from independent transformations display green
fluorescence specific to the guard cells.  No other green fluorescence is
observed in the transformants; no green fluorescence is observed in any
cell type in the controls.  We have tried both FITC on the fluorescent
microscope and confocal imaging and only find this guard cell specific
signal. This cell specificity has surprised us.  Has any one else made a
similar observation or are we looking at some artifact?  Any comments from
people working with GFP in plants would be much appreciated.

Candace Waddell

Robert Lamarche, Systems Manager
Biology Dept.  McGill University
1205 Docteur Penfield ave., Room W2/2
Montreal, Quebec, Canada  H3A 1B1
Phone: (514) 398-6469  Fax: (514) 398-5005

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