Hi,
I tried the original GFP in FACS (oops, read "flow-cytometry")
without success (transient or stable expression in HeLa cells with a
cmv promoter). The new "Greenlantern" from GIBCO/BRL/Life
Technologies (really, how many names do they need?) works well. It
has a S65T mutation of the type described by Ropp et al. [Cytometry
21:309-17 (1995)], who show that the excitation at 488 nm is much
more efficient.
disclaimer: my only relationship with G/B/L-T is that of a (usually)
satisfied customer.
