>> Does anyone know what the lower limits for detection of GFP in
>> mammalian expression systems is?
>> Niswender KD, Blackman SM, Rohde L, Magnuson MA, and Piston DW (1995)
> Quantitative imaging of green fluorescent protein in cultured cells:
> comparison of microscopic techniques, use in fusion proteins and detection
> limits. J.Microsc 180(2):109-116.
>> Estimate is 1um for cytoplasmic wt GFP. Similar if GFP is part of a
> fusion protein.
>
The detection limits should be much lower for the brighter chromophore
variants of GFP.
-= Paul =-
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