Karen P Scott <kps at rri.sari.ac.uk> wrote:
>>The following is a reply Bob MacDonald sent me concerning the use of a
>fluorescent plate reader.
>>>It will take a plate reader of unusual sensitivity if it works at all.
>>...If one were to, say, remove the cells from a 6 cm dish and put
>>them all in one well of a 96 well plate, it might work.
I believe that some (probably more expensive) plate readers will
concentrate particulate samples, such as cells, in 96 well plates by
filtration and read the fluorescent signals from the resulting smaller
areas. This is an automated procedure and is claimed to enhance
sensitivity significantly. This might facilitate the type of
concentration that you suggest without the need for additional manual
sample processing.
