fluorescein bleaching

Robert Fuller fuller at bozo.scs.uiuc.edu
Tue May 14 21:47:41 EST 1996

jfmcdon at BIOSCI.CBS.UMN.EDU (John McDonald) writes:

>I would like to know if there is a procedure out there to chemically 
>bleach a fluorescent probe that is attached to a protein while 
>maintaining the integrity of that protein. The probe is fluorescein 
>isothiocyanate. My initial attmpts at oxidation and acylation failed 
>to bleach the fluorescence. Has anyone tried to do this or have an 
>idea that might work? Thanks a lot.

I'm not sure what you mean by maintaining the integrity of the protein
(apparently attaching the label is acceptable to you).  Perhaps anti-
fluorscein antibodies may be of help to you.  (Check out Molecular Probes)

Robert Fuller                         |  r-fuller at uiuc.edu
M.D./Ph.D. Candidate in Chemistry     |  expert (noun) definition: 
Dept of Chemistry/College of Medicine |   "X"   =  unknown quantity 
University of Illinois, Urbana  61820 |   spirt =  drip under pressure

More information about the Fluorpro mailing list

Send comments to us at biosci-help [At] net.bio.net