jfmcdon at BIOSCI.CBS.UMN.EDU (John McDonald) writes:
>I would like to know if there is a procedure out there to chemically
>bleach a fluorescent probe that is attached to a protein while
>maintaining the integrity of that protein. The probe is fluorescein
>isothiocyanate. My initial attmpts at oxidation and acylation failed
>to bleach the fluorescence. Has anyone tried to do this or have an
>idea that might work? Thanks a lot.
I'm not sure what you mean by maintaining the integrity of the protein
(apparently attaching the label is acceptable to you). Perhaps anti-
fluorscein antibodies may be of help to you. (Check out Molecular Probes)
Robert
--
Robert Fuller | r-fuller at uiuc.edu
M.D./Ph.D. Candidate in Chemistry | expert (noun) definition:
Dept of Chemistry/College of Medicine | "X" = unknown quantity
University of Illinois, Urbana 61820 | spirt = drip under pressure
