> Are the sequences for the new pEGFP, pEGFP-N1, and pEGFP-C1 vectors
> from Clontech available?
These sequences were submitted to GenBank 5 weeks ago but they have
not finished processing them yet. Even before GenBank releases the
sequences, you can reqested them by sending an Email to
tech at clontech.com.
> pEGFP is accession number U43284 with a F64L mutation.
As Gerard Rouwendal wrote, you can derive the sequence of the EGFP
gene by adding the F64L mutation to the sequence of Seed's humanized
GFP gene in phGFP-S65T (U43284). Note however, that the vector
backbone of pEGFP-1, pEGFP-C1 & pEGFP-N1 is completely different from
the phGFP-S65T backbone.
-= Paul =-
.~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~.
! Paul Kitts !
! CLONTECH Laboratories, Inc. !
! 1020 East Meadow Circle Email: pakitts at clontech.com !
! Palo Alto Phone: 415 424 8222 x1463 !
! CA 94303-4230, U.S.A. Fax: 415 354 0776 !
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