Dear all,
I will shortly be tagging two proteins with GFP. Both are mammalian
phospholipases which I want to transfect into cells together and
separately and visualise both. What I'd like to know is
1. Which two GFP mutants would you rate highest e.g. S65T, humanized ,
blue-shifted, red-shifted , etc
2. Would you recommend N-terminal tagging or C-terminal tagging,
3. Is there a difference in ease or efficieny of transfection between
kits/mutants
4. Taking these into consideration, which kit do you recommend?
and, finally, what would be the best way to perform co-localisation
studies with the GFP tagged proteins and antibodies targeted to other
proteins?
Thanks in advance,
Fraser
F.D.Brown at bham.ac.uk
