I would like to use a fluorescent protein as a marker for DNA uptake in
transient assay (mammalian cells) but need to counterstain the cells for
a second protein with FITC ie green! Is there a red or blue fluorescent
protein which is easy to see using a fluorescent microscope?
Secondly, I have tried using S65T with little success in COS and 3T3
fibroblasts. Fluorescence is there but is weak - we transfect with
sv40-driven S65T and 24 - 48 hours later look at the cells (growing on
slides) under FITC illumination - the cells are still in DMEM and are
viable. Can anyone give me a good protocol for using GFP?
Many thanks
G Atherton
reply to grggta at picr.cr.man.ac.uk
