We have a final year undergraduate student, who would like to use GFP in
S.cervisiae as a marker for chromosome stability.
Does anybody have GFP(preferably S65T, but not essential) expressed
from a good yeast promoter that gives a nice fluorescent signal. It isn't
a problem if it is a fusion protein that is localised to a cell structure,
but if it is localised it must be easily seen without too powerful a
magnification (x20 or x40 objectives).
It would be much appreciated if you were willing to share this
construct with for the purposes of this project.
Thanks alot
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Pete Corish,
CRC Chromosome Mol.Biol.Group,
Dept. of Biochemistry,
University of Oxford,
South Parks Road,
Oxford OX1 3QU
Tel:- 01865 275222
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