Baulcombe et al. (Plant J 7: 1045-1053 [1995]) report that in PVX-GFP
infected cells 'The nucleus was the most intensely fluorescent structure
in the cell...'. Similarly, using fluorescence microscopy we can readily
distinguish mesophyll cells of transformants expressing the gfp gene
under the control of the E35S promoter from control cells; in fact it is
the clearest and most characteristic difference between them. Whether
this phenomenon has anything to do with sequences promoting GFP
translocation across the nuclear envelope as suggested by Baulcombe et al
. in the abovementioned paper remains to be seen.
--
Gerard J.A. Rouwendal
Tel. +31-(0)317-475315/Fax. +31-(0)317-412260
Agrotechnological Research Institute ATO-DLO
P.O. Box 17, 6700 AA Wageningen, The Netherlands
