>> Hi, I was wondering if anyone working with gfp in bacterial cells has
>experienced the problem which is driving me insane at present.
>>I previously had bacterial cells which expressed the gfp construct very
>strongly and the cells were highly fluorescent when examined
>microscopically, exciting them at the appropriate wavelength. However the
>SAME cultures seem to have lost a substantial amount of the fluorescence and
>from visibly seeing >90% of cells fluorescing I can now see <10%, although I
>can still see the nonfluorescent cells. I have tried an activity dye which
>seems to indicate that all the cells are viable so was wondering if anyone
>has any idea how to overcome this problem.
>>Thanks in advance, Dr Karen P Scott.