I would very much appreciate any comments on the use of mutated
fluorescent protein (s65t or y66w) together with fura-2
Would the emission of the GFP photobleach fura?
What is the percentage of s65t or y66w emmision at 340 and 380 versus
fura?
thank you for your help
Cecile
cfavre at umabnet.ab.umd.edu