On Mon, 4 Aug 1997, Jamie White wrote:
>> I would recommend trying the Clontech EBFP, actually. The catalog number is
> 6068-1. More information is on their web site (http://www.clontech.com/).
> I have had good results with their pEGFP vectors.
>> Sorry I can't be of more help. I'll be surprised if anyone gets BFP to work
> very well. I've been fooling with it for quite a while, with not very good
> results.
We just bought the mammalian BFP expression vector pQBI50-BFP from Quantum
Biotechnologies (Laval, Quebec) which features F64L, Y66H, V163A, a CMV
promoter, neoR, and an Nhe I site just downstream from the ATG useful for
creating fusion proteins. We'll transfect it this week and let you know
how things go. I was attracted to the nice Nhe I site useful for fusing
gfp to the C-termius of cellular proteins. We bloody hope we can get it
working, 'cause our real aim is FRET.
