Hello,
I am hoping to use GFP as a marker for gene activity in specific cell
layers of chimeric plants. We have no problem seeing mGFP5 in the
root (and petals), but cannot resolve GFP fluorescence from background
autofluorescence in the leaves, sepals and other chlorophyll
containing organs. Is there a special filter to use on a standard
microscope, or apparatus (other than confocal) which would allow
quick characterization?
Thanks in advance for your suggestions,
Natasha Myers
Sieburth Lab
McGill University
Montreal, PQ
