Dear Natasha,
from what your saying I guess you are using a filter set with a longpass
emission filter, which then leads to detection of chlorophyll
autofluorescence in green tissue. So you might look around for a bandpass
(emission) filter set which will exclude the red autofluorescence.
Christoph
>Hello,
>I am hoping to use GFP as a marker for gene activity in specific cell
>layers of chimeric plants. We have no problem seeing mGFP5 in the
>root (and petals), but cannot resolve GFP fluorescence from background
>autofluorescence in the leaves, sepals and other chlorophyll
>containing organs. Is there a special filter to use on a standard
>microscope, or apparatus (other than confocal) which would allow
>quick characterization?
>>Thanks in advance for your suggestions,
>>Natasha Myers
>>Sieburth Lab
>McGill University
>Montreal, PQ
Christoph Reichel
The Scripps Research Institute
Dept. Cell Biology, BCC 206
10550 North Torrey Pines Rd.
La Jolla, CA 92037
U.S.A.
phone: (619) 784-2918
fax: (619) 784-2994
