Alex,
Julie is right. Fully denatured GFP doesn't fluoresce. However, according to
Clontech's Application Notes fluorescence can be observed on native or 1%
SDS gels (Inouye & Tsuji, 1994).
Paul
On 12 Aug 1997, Alex Chang wrote:
> I am planning to do immunoprecipitation of my GFP-tagged protein, after
> SDS-PAGE and transblotting onto a membrane, look at the GFP-tagged protein
> directly by a UV light source without using antibody detection (or simply
> look at the gel directly without transblotting).
>> I'd like to know anyone has done this, and what kind of UV source can be
> used.
>>> Alex Chang
