I am planning to do immunoprecipitation of my GFP-tagged protein, after
SDS-PAGE and transblotting onto a membrane, look at the GFP-tagged protein
directly by a UV light source without using antibody detection (or simply
look at the gel directly without transblotting).
I'd like to know anyone has done this, and what kind of UV source can be
used.
Alex Chang
Pathology
University of British Columbia
achang at hivnet.ubc.ca
