We made a pEGFP stable cell line just by transfecting then selecting with
G418. The only thing that I would really make sure of is that when you
made your pEGFP construct you pay close attention to the fact that
bacterial resistance gene in the plasmid is Kanamycin NOT ampicillin.
Debs
P.S. the transfection was done with lipofectamine.
On Thu, 13 Feb 1997, Jean-Philippe Hugnot wrote:
[snip] about stable cell line with pEGFP
> Hugnot J.P.
> Institute of Psychiatry
> Department of psychology
Schuback at helix.mgh.harvard.edu
Lab Manager/Senior Tech
Massachusetts General Hospital
Molecular Neurogenetics
