Dear GFP-ers,
I would like to find out a few things about gfp, or the new modified
versions of gfp such as the ones sold by Clonetech.
I plan to express a gfp fusion protein where a nuclear protein is fused
to the Nterm of gfp. I plan to express this in the mouse and FACS sort
live cells obtained from such mice.
1. does anyone have any experience with FACS sorting of gfp expressing
cells ? Does it work with mesenchymal embryonic cells ?
2. is there a convenient source of gfp DNA that would have the following
structure:
multiple cloning site (MCS)-gfp- poly A addition site - MCS
3. does this group have a FAQ ? If so, where could i find it ?
Thanks for your help,
Artur Kania
