In article <33090AEC.BFF at columbia.edu>, ak316 at columbia.edu wrote:
> Dear GFP-ers,
>> I would like to find out a few things about gfp, or the new modified
> versions of gfp such as the ones sold by Clonetech.
> I plan to express a gfp fusion protein where a nuclear protein is fused
> to the Nterm of gfp. I plan to express this in the mouse and FACS sort
> live cells obtained from such mice.
>> 1. does anyone have any experience with FACS sorting of gfp expressing
> cells ? Does it work with mesenchymal embryonic cells ?
>
The EGFP-Mutatation from Clontech was found by FACS. The procedure is
described in the "Clontechniqes" April 1996, also available at their
Website
(http://www.clontech.com/clontech/Updtoc.html)
> 2. is there a convenient source of gfp DNA that would have the following
> structure:
> multiple cloning site (MCS)-gfp- poly A addition site - MCS
I' guess all the commercial products should have that. The structure of
two GFP's e.g. is described in the same issue of "Clontechniques"
>> 3. does this group have a FAQ ? If so, where could i find it ?
>
Not to my knowledge, but there is the general bionet archive, where you
can find older messages from the newsgroup:
http://www.bio.net:80/hypermail/FLUORESCENT-PROTEINS/
There is also a searchable index.
Greetings
Steffen Dietzel
--
Steffen Dietzel
Zentrum fuer molekulare Biologie Heidelberg (ZMBH)
University of Heidelberg
Im Neuenheimer Feld 282
69120 Heidelberg, Germany
Phone: +49/6221/54-6836 or -6879. Fax: -5893
listservmail:dietzel at sun0.urz.uni-heidelberg.de
personal mail: dietzel at wotan.iwr.uni-heidelberg.de
