Is anyone using Clontech's pEGFP reporter vectors? I want to compare
expression of the F64L/S65T double mutant to the same with "humanized"
codons. Clontech claims the change in codon usage results in around a
7-fold increase in fluorescent signal (in CHO cells) - can anyone verify
this (in any cell type)? I'm using GFP as a reporter in chicken
blastodermal cells.
Thanks for any input.
Gord Speksnijder
Department of Genetics
B410 Life Sciences
University of Georgia
Athens, GA 30602-7229
phone (706) 542-1420
fax (706) 542-1413
email gord at bscr.uga.edu
