Counting GFP colonies

Hernan Espinoza espinoza at cgl.ucsf.edu
Mon Jan 6 17:24:52 EST 1997

pboucher at UMICH.EDU (Paul Boucher) writes:

>I am interested in counting colonies that stably express GFP for 
>cytotoxicity assessment studies.  I want to be able to count colonies 
>that lack and express GFP.  Is there an easier way of doing this than 
>counting the colonies under a fluorescent microscope?  Perhaps some sort 
>of fluorescent dish scanner.  I would appreciate any input.  Thank-you.

	Do you need to count colonies, or would counting cells work?
If so, you could use a FACS machine to count up cells expressing and
not expressing GFP.  It could give you better numbers and the you
would only have to handle a few tubes instead of many plates.
Well, that's my lame suggestion.  -Hernan

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