Like Dr. David Roof, I would also like to know whether GFP spectra change
upon fusion to proteins and whether there is a difference between N- and
C-terminal fusions in this regard ?
As a scientist working on lysosomal proteases, I am, in addition,
interested in any quenched GFP constructs that might be useful for further
developments into specific protease substrates.
Thank you for your input,
Daniel Keppler, PhD
Department of Pharmacology
Wayne State University School of Medicine
540 E. Canfield Avenue, Scott Hall 6306
Detroit, MI 48201
Tel.: (313) 577-0514
Fax: (313) 577-6739