MPalacios wrote:
>question: has anyone dtermined the 1/2 life of GFP protein?
> eg: if my
>vector was transiently induced and inhibited sending a bolus of GFP ..how
>long will that GFP last?
This seems to be highly dependent on the system/cell type. I've
heard/read figures ranging from 30 minutes up to several days. Also, if
GFP is used in a fusion construct, it's halflife can be strongly
influenced by that of the fusion partner.
>Clontech says that a transient expression is on
>the order of something like 2-3 days.
This will probably depend on the construct. In all the cell lines I've
used so far (quite a few), transient GFP expression from our beta-actin
promotor driven vectors lasted for at least a week (for CHO, 3T3, Hela,
etc., i.e. until the cells died) up to more than six weeks (e.g. in
primary hippocampal cells).
Anyway, my comments won't really help you, I'm afraid. I propose that you
just give a try.
Beat
-----------------------------------------------------
Dr. Beat Ludin, FMI, Maulbeerstr 66, 4058 Basel, Switzerland
Tel. +41 61 697 6697 / FAX +41 61 697 3976
Internet:ludin at fmi.ch / Compuserve:100102,1527
