Hello fellow colleagues.
I am trying to express a EGFP-fusion in muscle and monitor this expression
with the 41017 filter from Chroma(exi:470/40, emi:525/50, dicro:495LP). I
do not think this filter is optimal for EGFP-imaginig.
Does anyone have an idea regarding the best optical data for an EGFP filter
set and from which company it can be obtained. I know that the optimal
filter set might change with type of cells used, type of fusion etc.
In addition I would like to ask if anybody know where it is possible to get
a GFP vector containing a muscle specific promoter.
Any help will be highly apriciated.
Regards
Hans.P.P.M.Lauritzen
MFI Bygn. 12.4
Panum institutet
Blegdamsvej 3 C
2200 Copenhagen N
Denmark.