IUBio

GFP extraction proceedure

S J Hunter BOP96SJH at shef.ac.uk
Wed Apr 1 08:08:19 EST 1998


Hi,

Is there an suitable method/buffer for extracting GFP5-er from plants.  
Maximising the amount of GFP obtained but minimising the amount of chlorophyll 
in the soluble extract,as chlorophyll results in the quenching of my GFP 
signal.

Any advise would be appreciated

Thamks Jayne




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