GFP and Paraffin/Frozen Sections

Andrew Brenner abrenner at ieo.it
Wed Apr 1 09:34:05 EST 1998

Dear Everyone,

I am generating reporter mice with GFP and I am facing the problem as
how to fix the cells.  How well does GFP hold up in formalin fixation
and paraffin embedding?  Also, if paraffin embedding is not possible,
can anyone suggest some alternatives that will allow analysis of GFP in

Thanks for any and all help.
Andrew J. Brenner, Ph.D.
Department of Experimental Oncology
European Institute of Oncology
Milan  Italy  20141
39 2 5748 9871   FAX 5748 9851

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