Guito <tremblgu at medcn.umontreal.ca> writes:
>Hi,
>There seems to be a myriad of GFP mutants.
>I just heard about a fast-folding mutant of GFP that seems to be fit for
>the experiments we intend to do in E.coli.
>We allready have a more soluble and red shifted mutant (smRSGFP), but a
>fast-folding GFP would be great in addition to the mutations of smRSGFP.
>Can anyone point me towards a reference about that fast-folding mutant
>please?
>------------------------------
>Guy Tremblay
>tremblgu at magellan.umontreal.ca
Guy,
Hugh Fisher in our lab at Rutgers has come up with a GFP variant which forms
much more quickly than wild type and under oxygen limiting conditions. His
e-mail is kawasaki at rci.rutgers.edu. Please contact him for some DNA if you
are interested.
Daniel Gonzalez
Rutgers University