EGFP vs. emerald

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Tue Dec 15 22:56:59 EST 1998

In article <367705CB.D0839E6 at hsc.usc.edu>, logg at hsc.usc.edu wrote:

> Hi.  If anyone's interested, I've compared the relative brightnesses of
> EGFP (Clontech) and emerald GFP (Packard).  In three different
> retroviral vector constructs, the emerald gives a notably brighter
> signal, both by flow cytometry and by fluorescence microscopy.  The EGFP
> and emerald vectors I used are identical save for the 4 point mutations
> that distinguish the two GFPs.  So, if anyone's having trouble detecting
> their GFP, using emerald might help.
> Chris
> logg at hsc.usc.edu

How stable is the emerald plasmid?  Several of us have had trouble
with pEGFP and so have been using Gibco's pGreen-Lantern as it
is easier to propagate in bacteria.
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA

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