In article <367705CB.D0839E6 at hsc.usc.edu>, logg at hsc.usc.edu wrote:
> Hi. If anyone's interested, I've compared the relative brightnesses of
> EGFP (Clontech) and emerald GFP (Packard). In three different
> retroviral vector constructs, the emerald gives a notably brighter
> signal, both by flow cytometry and by fluorescence microscopy. The EGFP
> and emerald vectors I used are identical save for the 4 point mutations
> that distinguish the two GFPs. So, if anyone's having trouble detecting
> their GFP, using emerald might help.
> Chris
>logg at hsc.usc.edu
How stable is the emerald plasmid? Several of us have had trouble
with pEGFP and so have been using Gibco's pGreen-Lantern as it
is easier to propagate in bacteria.
Bernard
--
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA
