immunolabel of GFP-expressing drosophila embryos

[Andre Klarsfeld]

Hello,
I'm having trouble immunolabeling drosophila embryos that express GFP.
Specifically, the heptane-treatment required for fixation before antibody
is added appears to destabilize GFP. The better the antibody labelling, the
weaker the remaining GFP signal. Any suggestions welcome !
Andre


_______________________________________________
Andre Klarsfeld
"Genetique moleculaire des rythmes circadiens"
CNRS Inst. A. Fessard
91198 GIF-SUR-YVETTE cedex
tel : 33-(0)1 69 82 43 04
fax : 33-(0)1 69 07 05 38
email : Andre.Klarsfeld at iaf.cnrs-gif.fr

http://www.cnrs-gif.fr/iaf/iaf3/equipe3.html