On 28 Dec 1998 20:05:36 GMT, David N. Levy (levy at uab.edu) wrote:
> One more question: GFP fluoresces fine without the first methionine
> (which I suppose would be cleaved anyway followoing translation),
> but does GFP need the valine at position 2 for fluorescence?
David,
GFP does not need the Val at position 2 to fluoresce.
The second amino-acid in wtGFP is actually Ser: Met.Ser.Lys...
A Val was inserted in EGFP to create the full Kozak consensus
sequence around the ATG: Met.Val.Ser.Lys....
-=Paul=-
""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
" Paul Kitts, Ph.D. NIH/NLM/NCBI "
" Email: kitts at ncbi.nlm.nih.gov Bldg 38A, 8N803 "
" Phone: 301 435-6091 8600 Rockville Pike "
" Fax: 301 480-9241 Bethesda, MD 20894, U.S.A. "
""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""
