IUBio

EGFP microscopy

Beat Ludin ludin at FMI.CH
Wed Jul 8 10:02:17 EST 1998


rdudley at aherf.edu wrote:

>You don't have rubber cement in Sweden?  It's in every school supply section
>in every store here (USA).  If you don't like molten agarose, I wouldn't
>recommend rubber cement.  Despite what people have said about nail polish, it
>worked well for us in the past.

Nail polish works fine, but you may have to try several brands. We had 
one which effectively killed GFP fluorescence with a pretty long range 
(3-4mm). I guess it's a question of the solvent used and how well it 
diffuses into or mixes with the aequous mounting medium. 



-----------------------------------------------------
Dr. Beat Ludin, FMI, Maulbeerstr 66, 4058 Basel, Switzerland
Tel. +41 61 697 6697 / FAX +41 61 697 3976




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