CHK <chk108 at PSU.EDU> wrote:
> I am trying to find a way to fix a cell that has been bombarded with a
> GFP/MAP4 construct that is fluorescing for immunolocalization. The fixer
> has to sustain the fluorescent signal for approx. 12 hrs or more. If you
> know any, please let me know.
I have been rather successful with 4% Paraformaldehyde in PBS. If kept
in the fridge, the GFP-transfected cells still show nice fluorescence
the next morning. (I didn't check after longer time intervals.)
--Cornelius.
--
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany email: phak004 at rzbox.uni-wuerzburg.de SP4 */
/* "Science is the game we play with God to find out what His rules are." */
