Steffen Dietzel <s.dietzel at pop.life.uiuc.edu> wrote:
> To be able to do 3D data collection on our GFP-labeled cells we need to fix
> the coverslip somehow on the slide. Normally nailpolish is used to do this.
> However all those that we've tried decreased the fluorescence of the GFP. Has
> anybody an idea what to use instead?
>> Thanks in advance for any hints
>> Steffen Dietzel
Steffen,
I've tried a number of methods for live cells (nail polish, agar, pva)
and none really helped. In the end I got an 'Autofluor Cell Chamber'
from Molecular Probes, mounted the coverslip in that, filled it up with
medium and put a 3.5cm petri disk lid on top (excluding any air
bubbles).
The whole assembly is them inverted (if using an upright microscope
rather than inverted) and mounted on the Confocal Mic stage.
For fixed cells I just mounted in Moviol.
--
Tony P Hodge
tph at mrc-lmb.cam.ac.ukhttp://131.111.84.120/tph/http://131.111.84.120/tph/psion/
