Gerald W. Gordon wrote:
> I don't know specifically for GFP but in general sealing of
> coverslips you can use valap which is vaseline, lanolin, and
> parafin (wax) in proportions to provide the degree of rigidity
> required.
<high tech application method snipped>
> The low tech people just heat a spatula in
> a bunsen burner and dip into the bowl of valap and let the
> molten valap run off onto the edge of the cover slip.
I've always used a small cotton swab left in a small beaker of
the stuff on a hot plate.
> I would expect the ingredients to be inert enough not to
> affect the GFP fluorescence.
It doesn't seem to affect the fluorescence at all; I've
used it for live cells and it works fine.
--
John Lye
e-mail to: rjl6n at Virginia.EDU
homepage: http://avery.med.virginia.edu/~rjl6n/homepage.htm
