Molecular Probes sells a rabbit anti-GFP polyclonal which works
on acetone and on methanol fixed tissue. I have found it to be
extremely specific, with a conjugated secondary it is almost as
bright as plain live GFP.
Janet Duerr
Program in Cell and Molecular Biology
Oklahoma Medical Research Foundation
In article <199803111244.MAA13330 at intwww.zit.med.uni-tuebingen.de>, wgschech at med.uni-tuebingen.de ("Wolfgang Schechinger") writes:
> Hi all.
>> Since acetone will kill GFP fluorescence: How will I get the
> cytoskeleton of 293 fibroblasts and smooth muscle cells stained?
> Rhodamine-phalloidin and coumarin-phalloidin work well on acetone
> fixed cells, but in methanol - nope. How might I get out of this
> dilemma? Would adding the phalloidin dye to the culture medium help,
> depite it will hurt cells?
> Could I use another fixing procedure, actin dye or what should I do?
>> Standing by for your suggestions!
>> Wolfgang
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> Wolfgang Schechinger
> University of Tuebingen
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