Hi,
I know this question has been asked a thousand times, but here is it
again.
What fixative procedure can preserve the EGFP fluorescence and in the
meantime suitable for immunohistochemistry with another antibody? I don't
mind taking pictures of the EGFP fluorescence first before the
immunohistochemistry procedure. I just need to show EGFP and
immunohistochemistry on the same cells (on culture dishes).
Thanks in advance.
Chris Lau
_____________________________________________________________________________
Chris Lau, Ph.D. Tel: 415 476-8839
Division of Cell and Developmental Genetics FAX: 415 502-1613
Department of Medicine, VAMC-111C5
University of California, San Francisco E-mail : clau at itsa.ucsf.edu
4150 Clement Street
San Francisco, CA 94121