GFP gene number vs. fluorescence

Eugene ronlab at uic.edu
Thu Mar 12 14:35:49 EST 1998

ara wrote:

> Would you tell me how many GFP gene (pEGFP-C1) do transfected cells need
> for us to detect fluorescence? (I used CMV promoter and HeLa as
> transfected cell : I don't mind any cell type :) )
> I think the transfected cells should need over 1 number in order to
> satisfy our dectection.

Indeed, cells are readily detectable with a single copy of CMV-driven EGFP.

> Thanks in advance..

You are very wellcome!

Eugene Kandel.
U09577 at uic.edu

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