Hi,
Did anyone notice heterogenous expression of EGFP in monoclonal cell
population?
We observed that even monoclonal NIH 3T3 cells stably transfected with
pEGFP-C1 showed heterogenous expression of EGFP. This led to less
percentage of postive(green) cells in a population on FACS.
Does anyone know if this is usual? Does anyone know how they could achieve
near 2 log level increase of fluorescence intensity in a population?(Shown
by the right shift of transfected cells compare to wild-type in FACS
histogram in published papers). Is this achieved by constantly sorting the
cells?
Thanks in advance for any suggestion.
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Yuan Shen
Dept. of Microbiology/Immunology
Roswell Park Cancer Institute
State University of New York at Buffalo
