Hi,
I have the same problem with the cell line I use: I have heterogeneous
fluorescence, even if I work with a monoclonal population. In this
population, the cells are stable for GFP and always in culture media with
G418. I think that is very difficult (may be impossible) to obtain a
population stable for GFP, that express every time a good and homogeneous
level of GFP.
I think it's usual.
Sincerely,
Sebastien PARIS
___________________________________________
INSERM U295
Faculte de Medecine-Pharmacie de Rouen
Avenue de l'Universite
B.P. 97
76803 St-Etienne-Rouvray Cedex
France
Tel : (33)-02-35-65-52-74
Fax : (33)-02-35-66-12-78
E-Mail : nathalie.porchet at univ-rouen.fr
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