Eugene Kandel. wrote:
>I would suggest to look up the paper or, at least, the abstract
>before
>using it as an example of a failed GFP fusion.
E.Kandel is absolutely right.
T.Aoki et al.
were treating GFP-protein A with heat and observed the loss of
fluorescence and the electrophoretic migration shift. This means that
their fusion is indeed a success (if one measures a success in
fluorescence AU) but a thermolabile one.
My apologies...