If you follow the unfluorescent cells constantly, you will find that those
cells aren't fluorecent all the time instead of cell cycle dependent. THe
half life for GFP is quite long. If your protein is not secreted, I
believe it will fluorescent in a cell cycle independent way.
YuBao WANG
Department of Biochemistry & CMBGT
Loma Linda University
Loma Linda
CA 92354
