Anti-Fade for GFP

[Wolfgang Schechinger]

<snip snip>
Science 217, 1252-5 (1982) says 5% n-Propyl gallate in glycerol does 
the job. Of course it then was not tested on GFP.
To me, it seems to preserve S65T fluorescence quite well.

However, I recently tried Permafluor embedding 
medium from Immunotech (Marseille, France) with initially very good
results. But when I examined the slides two weeks later, I observed a 
high bacjground fluorescence with FITC filters. H33258 (bisbenzimide) 
in contrary was looking quite well. 
Was it wrong to store the slides at RT? Would they better be kept in 
the fridge?

Wolfgang

-----
This message is RNAse free - please don't touch!
-----                              
Wolfgang Schechinger         
University of Tuebingen
email: wgschech at med.uni-tuebingen.de
http://www.medizin.uni-tuebingen.de/~wgschech/research.htm
-------
!Junk mail is *not* appreciated!
SPAMs will be answered automatically by sending my 128MByte virtual memory file.
This is *NO* joke.
------
usual disclaimers apply
------