Inducible GFP in E.coli

'Mike' Michael J. Moser moser at U.WASHINGTON.EDU
Fri Mar 27 14:16:03 EST 1998


I have used a plasmid that has S65T-GFP cloned into one of the T7
(Studier) vectors.  However it is quite leaky and GFP fluorescence is
easily detected even in the absence of IPTG.


Mike Moser                                            Tel: 206-543-6585
UW Department of Pathology                            FAX: 206-543-3967
Box 357705                                       moser at u.washington.edu
Seattle, WA  98195                 http://weber.u.washington.edu/~moser

On 27 Mar 1998, Dr.Narayana R.Isola wrote:

> I am looking for GFP clones in E.coli plasmids with inducible promoters,
> IPTG will be fine for now.  Any have any information please contact me
> Narayana R.Isola Ph.D.,
> Oak Ridge National Laboratory,
> tel 423 574 5893
> fax 423 576 2115
> e-mail isolan at ornl.gov

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