Narayana
I have used a plasmid that has S65T-GFP cloned into one of the T7
(Studier) vectors. However it is quite leaky and GFP fluorescence is
easily detected even in the absence of IPTG.
MM
Mike Moser Tel: 206-543-6585
UW Department of Pathology FAX: 206-543-3967
Box 357705 moser at u.washington.edu
Seattle, WA 98195 http://weber.u.washington.edu/~moser
On 27 Mar 1998, Dr.Narayana R.Isola wrote:
> I am looking for GFP clones in E.coli plasmids with inducible promoters,
> IPTG will be fine for now. Any have any information please contact me
>> Narayana R.Isola Ph.D.,
>> Oak Ridge National Laboratory,
>> tel 423 574 5893
>> fax 423 576 2115
> e-mail isolan at ornl.gov>>>
