Steffen Dietzel <s.dietzel at pop.life.uiuc.edu> wrote:
> I am looking at mammal cell culture cells expressing GFP. To facilitate
> observation of cells in the fluorescent microscope, I would like to use an
> Anti-Fade reagent that minimizes bleaching. Currently we have Propyl Galate
> solved in 90% Glycerol in the lab, however it quenches the GFP-fluorescene in
> formaldehyde fixed cells pretty much!
Are you sure that it is the Propyl Galate? When I mount GFP-expressing
cells in Propyl Galate without fixing, I get a lot of fluorescence;
however, when I first fix them in 4% paraformaldehyde, fluorescence is
almost completely lost :-(
Any hints appreciated...
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany email: phak004 at rzbox.uni-wuerzburg.de SP4 */
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