We tried the same IRES-EGFP vector in HeLa cells and saw real good EGFP
expression, approximately 50% cells were fluorescent. We used BRL
TransfectAMINE plus kit for our transient transfection experiment. EGFP
expression was detectable from 24 -76 hours after addition of DNA with the
same % of positive cells.
_________________________________________________________________________
On 28 Mar 1998, Erick Noensie wrote:
> I am using the Clontech IRES-EGFP vector. I know that the IRES is
> attenuated, but I can't see anything glowing in Hela or mouse cells. I also
> did controls for transfection so I dont think thats the problem. Has
> anyone else had this problem or have any insight into this ?
>> Thanks.
>>>> Erick Noensie
> Johns Hopkins University School of Medicine
>>>
_____________________________________________________________________________
Chris Lau, Ph.D. Tel: 415 476-8839
Division of Cell and Developmental Genetics FAX: 415 502-1613
Department of Medicine, VAMC-111C5
University of California, San Francisco E-mail : clau at itsa.ucsf.edu
4150 Clement Street
San Francisco, CA 94121
