Dear Erick,
Yes, we have found the same thing in ES cells. We have good rates of
transfection and loads of bright green cells with the original EGFP vector
from Clontech, but much reduced signal (about 90-95% less) in IRES-EGFP
(using the same promoter). We would also welcome any comments.
Viv
>I am using the Clontech IRES-EGFP vector. I know that the IRES is
>attenuated, but I can't see anything glowing in Hela or mouse cells. I also
>did controls for transfection so I dont think thats the problem. Has
>anyone else had this problem or have any insight into this ?
>>Thanks.
>>>>Erick Noensie
>Johns Hopkins University School of Medicine
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Dr. Vivienne S. Marshall, Wisconsin Regional Primate Research Center,
University of Wisconsin, 1223 Capitol Court, Madison, WI 53715, USA
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