Sharon Barr wrote:
>One of my coworkers is trying to localize a protein in cultured mammalian
>cells using BFP. She finds that the blue photobleaches almost immediately.
>I do not see the same effect with GFP. I was referring to this rapid
>photobleaching when I said instability.
Ok, so the answer is yes. BFP does bleach a lot faster than GFP
(especially the "red-shifted" variety like GFP(S65T), EGFP, etc.).
Beat
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Dr. Beat Ludin, FMI, Maulbeerstr 66, 4058 Basel, Switzerland
Tel. +41 61 697 6697 / FAX +41 61 697 3976
