Wouldn't it be useful to dissect signal transduction pathways to have a
peptide substrate (or peptide substrate mimetic) which would emit an
altered fluorescence (band-shifted) depending on its state of
phosphorylation?
For example, a substrate for a MAP kinase which might fluoresce at 480 nm
when unphosphorylated and at 570 nm when phosphorylated?
I would imagine that such a substrate has been imagined, but I have not
been able to find it in the literature.
Reverse precedents exist in a manner of speaking, substrates for
phosphatases which emit light (luminescence) when the phosphate is removed
(by a phosphatase). For example, CDP-star from Tropix, Inc:
http://www.tropix.com
a company which also has luminescent substrates for b-gal and
beta-glucuronidase.
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Anton Scott Goustin, Ph.D. * * *
Center for Molecular Medicine and Genetics *
Wayne State University * *
5047 Gullen Mall, Room 5167 *
Detroit, Michigan 48202-3917 USA *
TELEPHONE 313-993-7688 * * *
FAX 313-577-6200 * *
E-mail asg at cmb.biosci.wayne.edu *
WWW http://cmmg.biosci.wayne.edu/asg/asg-home.html
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"IT IS KNOWN THAT NATURE WORKS CONSTANTLY WITH THE SAME MATERIALS. SHE
IS INGENIOUS TO VARY ONLY THE FORMS." --E. Geoffroy Saint-Hilaire, 1807
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